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Technology platform Protein expression To permit structural studies milligram quantities of protein are required. We have improved current technologies and can now offer eukaryotic membrane and soluble proteins in milligram amounts. For our purposes host-vector systems are exploited utilizing both mammalian cells and insect cells. This technology allows to produce fully functional proteins with post-translational modifications similar to those of the native protein.NMR requires that protein is labelled with stable isotopes. Mammalian and insect cells require complex mixtures of nutrients, including at least essential amino acids. We are addressing this issue by developing a cost-effective procedure to allow stable isotopically labelling of proteins on a preparative scale. This is achieved by using labeled biomass from various microbiological sources. PLI also provides various metabolic precursors for labeling of microorganisms (algal, bacterial, yeast, insect, mammalian). In addition, tailor made labelling schemes can be designed using either modular organic chemistry or biotechnological approaches.  |
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